Artigos Médicos

Physiological and molecular characteristics of carbapenem resistance in Klebsiella pneumoniae and Enterobacter aerogenes


Introduction: Bacterial resistance is a growing concern in the nosocomial environment in which Klebsiella pneumoniae and Enterobacter
aerogenes play an important role due to their opportunism and carbapenemase-production. This work aimed to evaluate physiological and
molecular characteristics of carbapenem-resistant K. pneumoniae and E. aerogenes isolated in a Brazilian tertiary hospital.
Methodology: In total, 42 carbapenem-resistant bacteria isolated from clinical specimens were included (21 K. pneumoniae and 21 E.
aerogenes). Drug-sensitive K. pneumoniae (n = 27) were also included. Antimicrobial susceptibility and biocide tolerance patterns, hemolytic

activity, tolerance to oxidative stress, and aggregative ability were assessed. Genetic markers related to carbapenem resistance, or ESBL-
production were screened by PCR.

Results: Compared to drug-sensitive strains, carbapenem-resistant K. pneumoniae were more tolerant to biocides and to oxidative stress, and
they displayed an increase in biofilm formation. The genetic markers blaKPC (95.2%) and blaTEM (90.5%) were the most frequent. Among the
carbapenem-resistant E. aerogenes strains, blaKPC, and blaTEM were detected in all bacteria. Drug-sensitive E. aerogenes were not isolated in
the same period. blaSHV, blaVIM, and blaCTX markers were also observed among carbapenem-resistant bacteria.
Conclusions: Results suggest that carbapenemase-producing enterobacteria might show peculiar characteristics regarding their physiology
associated with their environmental persistency, virulence, and multidrug resistance. The observed phenomenon may have implications not
only for antimicrobial chemotherapy, but also for the prognosis of infectious diseases and infection control.


Keywords: Carbapenemases; enterobacteria; antimicrobial resistance; biocide tolerance.


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